伊犁小檗果实红色素超声提取及DPPH·自由基清除活性

摘要

以伊犁红果小檗果实为原料，在单因素试验基础上，利用正交设计优化了红色素的超声提取工艺，并研究了其色素对DPPH·自由基的清除效果。结果表明，最佳工艺：体积分数为60%的乙醇做提取剂，在50℃下超声提取15 min，料液比为1∶20（g∶mL）。色素得率为41.1%（脱籽）和24.7%（未脱籽），色价为0.665。红色素粗提取物对DPPH自由基有一定的的清除活性且清除率与浓度之间有量效关系。%　　The red pigment of Berberis iliensis Popov. fruit as research material, on the basis of single factor experiment, the ultrasonic-assisted extraction technique was optimized by orthogonal design. The results showed that the optimum extraction technology was using 60%ethanol, ratios of material to liquid by 1∶20(g∶mL), extracted at 50℃for 15 min, the yield of pigment was 41.1%( seedless) and 24.7%( seeded), its color value was 0.665, the red pigment had some scavenging activities on DPPH·with dose-effect relationship. Natural resources of Berberis iliensis Popov. was rich and its red pigment colour was bright. It had value of development and application.