芒果叶不同极性部位提取物的抗氧化活性

摘要

对芒果叶不同极性部位提取物的抗氧化活性进行研究。用DPPH法考察最优反应条件，综合评价其抗氧化活性，测定其黄酮含量，以IC50值作为评价清除DPPH自由基能力的指标，用芒果苷和抗坏血酸作为对照品进行自由基清除率的比较。试验结果表明：芒果叶石油醚、乙酸乙酯、正丁醇萃取物及水部位提取物的黄酮含量依次为：（33.616±3.208）、（292.063±1.100）、（180.469±2.067）、（21.717±1.157） mg/g，IC50值分别为：102.877、9.014、19.494、221.784μg/mL。由此可以看出，芒果叶各类提取物均具有一定的抗氧化活性，其中乙酸乙酯萃取物抗氧化活性最强，其次为正丁醇、石油醚，最后为水部位提取物。相关性分析结果表明，芒果叶各极性部位提取物的黄酮含量与清除DPPH自由基的能力呈负相关，芒果叶各部位提取物的黄酮含量是影响其抗氧化活性的主要因素。%Different polarity fractions of extracts from mango leaves were evaluated for their antioxidant activities. Study the optimal reaction conditions and comprehensive evaluate the antioxidant activities of the four parts by DPPH feel radical systematic method,tested the flavonoid content,take the IC50 values as the Evaluation of scavenging activities towards DPPH feel radical, compared with mangiferin and ascorbic acid. The results showed that the flavonoid content and IC50 values of mango leaves' petroleum ether part, ethyl acetate part, n-butanol part and water part were (33.616±3.208),(292.063±1.100),(180.469±2.067),(21.717±1.157)mg/g;102.877, 9.014,19.494,221.784 μg/mL. Each part of extracts from mango leaves have antioxidant activities, while the ethyl acetate part has the strongest effect on it. Then followed the n-butanol part, petroleum ether part, the water part is ranged in the last. The correlation analysis showed that the flavonoid content and scavenging activities towards DPPH feel radical were highly significant correlated.The flavonoid content were main factors for the antioxidant activities.