The appropriate extraction conditions of betalain from beets by single factor experiment (solvent ratio, temperature, extraction time, pH, extraction solvent) were determined. On the basis of that conclusion, using response surface methodology (RSM) for optimizing extraction of betalain investigated the effects of solvent/material ratio, microwave output power and micro-wave time. The results showed that among the three factors the microwave time most significantly affected on the absorbance , followed by the microwave output power and the solvent/material ratio. The optimal conditions were as follows:solvent/material ratio 1:5.76(g/mL), microwave output power 398 W and microwave time 112.8 s, the highest absorbance was 0.711 4. Through ultrafiltration and the stability was studied, research found that the sunshine and pH had significant impact on the stability. Different stabilizers have a certain improvement on the stability of betalain , and using of sodium benzoate (0.04%) and VC (0.04%) together can improve its stability significantly.%通过单因素试验对甜菜红色素提取条件(料液比、温度、提取时间、提取液pH、提取溶剂等)进行初步探究,并通过微波辅助对色素提取进行响应面优化设计,结果表明影响提取最显著的因素是微波时间,其次是微波功率,最后是料液比.最适提取条件:料液比1:5.76(g/mL),微波功率398 W,微波时间112.8 s,此条件甜菜红色素最大吸光度值为0.711 4.对提取的甜菜红色素进行超滤处理并对其粗提液进行稳定性研究,结果表明光照、pH对其稳定性影响显著,添加不同稳定剂对甜菜红色素稳定性有一定改善,苯甲酸钠(0.04%)和VC(0.04%)复配使用对于提高其稳定性作用较为明显.
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