植物饮料提取液中微生物的分离鉴定及耐热性研究

摘要

For studying on diversity and thermal resistance, 6 strains of microbe isolated from the plant bever-age extracts were identified by 16S rDNA sequence analysis and were measured D value, Z value by capillary tube method. The result showed that these isolates identified to be Bacillus stearothermophilus, Bacillus pumilus, Bacillus anthracis, Bacillus ginsengihumi, Alicyclobacillus sp. and Bacillus cereus, respectively. In addition, the results of thermal resistance of Bacillus stearothermophilus which was predominant thermoduric bacteria in canned food showed that the values of D121℃=1.4 min and Z=10.05℃.%以植物饮料提取液为研究对象，通过16S rDNA序列分析法和基于毛细管法测定微生物的D值和Z值的方法对分离出的耐热性菌株进行分子生物学鉴定和耐热性能研究。结果表明，本试验共分离出6株耐热性菌株，包括嗜热脂肪芽孢杆菌、短小芽孢杆菌、枯草芽孢杆菌、人参土芽孢杆菌、脂环酸芽孢杆菌和蜡样芽孢杆菌。其中，罐头食品中典型耐热性代表菌株嗜热脂肪芽孢杆菌芽孢的耐热性测试结果为：D121℃=1.4 min，Z=10.05℃。