HPLC-MS法测定罗汉果中10种氨基甲酸酯类农药

摘要

HPLC-MS was employed for determination of carbamate pesticides in Siraitia grosvenorii. The sam-ples were cleaned with solid-phase extraction column after ultrasonic extraction. The extracts were separated by gradient elution. The condition were VP-ODS column (2.0 mm×150 mm,5μm),temperature 40℃,speed 0.2 mL/min, inject 10μL. Pesticides were detected with multiple reaction monitoring mode. In 3 different degrees(250,500, 1 000μg/L),the average recoveries were 84.5%-111.7%,the relative standarddeviation were 1.8%-7.3%, the detection limits were 0.1μg/kg-2.0μg/kg. The method is accurate,rapid,anti-interference and suitable for determination of these pesticides in Siraitia grosvenorii.%建立HPLC-MS/MS分析方法，同时测定罗汉果中10种氨基甲酸酯农药残留量。样品经有机溶剂超声提取，提取液浓缩后经固相萃取小柱净化，采用梯度洗脱分离，色谱系统条件为VP-ODS色谱柱（2.0 mm×150 mm，5μm ），柱温40℃，流速0.2 mL/min，进样量10μL，以多反应检测模式监测。考察了3个水平添加下（250、500、1000μg/L），10种农药的平均回收率为84.5%~111.7%，相对标准偏差为1.8%~7.3%，检测限在0.1μg/kg~2.0μg/kg。该方法准确、快速、抗干扰能力强，可作为罗汉果中氨基甲酸酯类农药残留的测定。