萜类合成甲羟戊酸途径的关键基因的克隆及表达分析

摘要

【Objective】 To clone the gene length of the transcription factors in Salvia miltiorrhiza and analyze its initial expression and induced expression in Salvia miltiorrhiza.【Method】 Guided by high-throughput sequencing results and inspired from BHLH genes reported in arabidopsis and rice,full-length gene was cloned.We studied this particular BHLH gene,and analyzed intial expression and the induced expression on each part of Salvia miltiorrhiza.A 500bp sequence of SMbHLH-2 was cloned from the gen-omic DNA of Salvia miltiorrhiza.Through the ABA,MJ,AG ＋ and other biotic and abiotic inducible factor induction,we detected and analyzed the expression of the roots＇ during different induction time.【Result】 We found that increasing transcriptional activity of transcription factors might increase its transcription efficiency,which might increase the amount of secondary metabolites,thus improve the plant＇s medicinal active ingredient production.%目的：克隆丹参中普遍表达的转录因子基因全长,分析其在丹参各部位初始表达及诱导表达。方法：以深测序结果为指导,从拟南芥和水稻的已见报道的BHLH基因得到启发,克隆得到全长基因。比较其在丹参植株各个部位的不同表达量推测它与次生代谢途径的相关性,并通过ABA,MJ,AG＋等生物及非生物诱导因子的诱导,检测其在根部不同诱导时间的表达量情况,试图找出提高转录因子转录活性或转录效率,从而提高次生代谢产物得量,进而提高丹参作为药用植物的药用活性成分的产量。结果：得到转录因子基因全长,分析得出其在ABA,MJ,AG＋等作用下,不同时间段的表达情况。