玉米花粉复合酶解破壁工艺条件研究

摘要

Maize pollen are rich in nutrients and bioactive components. The wall-broken technology of maize pollen by composite enzy matic hydrolysis was investigated by orthogonal experimental design. The optimal technology conditions were enzyme concentration （ cellulose ： pectinase = 1 ： 1 ） 7.5% , temperature 50℃, pH 5.0, and time 9 h. Under the optimal technology conditions, the rate of wallbroken of maize pollen was 41.78%.%玉米花粉含有丰富的营养物质和生物活性成分。在单因素试验的基础上，利用四因素三水平正交试验设计，研究了玉米花粉复合酶解破壁的工艺条件。结果表明，玉米花粉复合酶解破壁的最佳工艺条件为：酶添加量为7．5％（纤维素酶：果胶酶=1：1）、酶解温度50℃、pH5．0、酶解时间9h，在此工艺条件下，玉米花粉破壁率为41．78％。