Pollen and leaf proteins after 2-D electrophoresis of the Aegilops geniculata × Secale cereale hybrids, amphiploids and parental forms

摘要

Proteins from pollen and leaves of parent forms and of a hybrid and an amphiploid Aegilops geniculata × S. cereale were subjected to a study by two-dimensional gel electrophoresis (2-DE). The majority of peptides found in the parents were revealed in the amphiploid pollen and the hybrid and amphiploid leaves, however, numerous parental peptides were absent both in hybrid and amphiploid. There were detected characteristic peptides in leaves of the hybrid as well as in the leaves and pollen of the amphiploid, which were absent in the material from both parents. 52 peptides were common for pollen and leaves of the amphiploid what corresponds to 14.2% and 25.7% of peptides in pollen and leaves, respectively. Three characteristic peptides were common for leaves and pollen of the amphiploid (MW 48.0 kDa in pH 5.9, MW 49.0 kDa in pH 6.9 and MW 22.0 kDa in pH 8.8).