动态高压微射流对猪胰脂肪酶的影响

摘要

The effect was studied of dynamic high-pressure microfluidization (DHPM) treatment on porcine pancreas lipase (PPL) enzyme activity and conformation.PPL was inactivated after DHPM treatment and the higher pressure, the less inactivationis.The relative activity increased during 24 h storage at 4 ℃.UV and CD spectra analysis showed, after DHPM treatment in 100 MPa, the UV absorption and β-sheet content reduced.Yet the UV absorption and β-sheet content increased with the increase of treat pressure.After 24 h storage at 4 ℃, the UV absorption and β-sheet content was further increased.The results showed that the relative activity was correlated with UV absorption and β-sheet content positively.%以猪胰脂肪酶(PPL)为原料,研究动态高压微射流(DHPM)对酶活性和构象的影响.结果显示:DHPM处理对PPL具有钝化作用,处理压力越大,钝化作用越小;4℃下贮存24 h,相对酶活有所回升.通过紫外光谱和圆二色谱分析DHPM对PPL构象的影响,发现经100 MPa DHPM处理后,PPL的紫外吸收强度降低,β-折叠含量减少,但随着处理压力增大,紫外吸收强度和β-折叠含量逐渐增大;4℃放置24 h后,紫外吸收强度和β-折叠含量进一步上升.PPL的相对酶活与紫外吸收强度、β-折叠含量呈一定的正相关.