高效液相色谱法分析武夷岩茶中的生物碱含量

摘要

选取10种典型武夷岩茶(5种肉桂和5种水仙)作为研究对象,以绿茶、铁观音和红茶为对照,通过高效液相色谱法检测样品叶片及冲泡茶汤中的可可碱、茶碱和咖啡碱含量,并依据各样品的生物碱含量进行主成分分析.试验结果发现,武夷岩茶叶片中的咖啡碱、可可碱和茶碱含量分别约占茶叶干重的2.341％,0.065％,0.011％,低于绿茶、铁观音和红茶;茶叶经过60 s沸水冲泡后,约46.56％,46.36％,50.15％的咖啡碱、可可碱和茶碱溶于茶汤中.此外,通过主成分分析发现,武夷岩茶的3种生物碱含量与绿茶、铁观音和红茶的生物碱含量有显著差异.%Wuyi Rock Tea is a kind of semi-fermented oolong tea,with unique flavor.The alkaloid content in the tea is associated with its flavor quality.In this study,10 typical Wuyi Rock Tea,including 5 Rougui and 5 Shuixian,were used to quantitative analyze the theobromine,theophylline and caffeine content in the tea leaves and tea infusions.Moverover,the alkaloid content in green tea,Tieguanyin Tea and black tea sample were also measured.Then,Principal Component Analysis (PCA) was utilized to further investigate the difference of the alkaloid content in all these tea samples.The results indicated that,the caffeine,theobromine and theophylline content in the Wuyi Rock Tea leaves were 2.341％,0.065％ and 0.011％,respectively,which were lower than those ingreen tea,Tieguanyin Tea and black tea.After immersed in boiling water for 60 s,approximate 46.56％,46.36％ and 50.15％of caffeine,theobromine,and theophylline were dissolved into the infusions.PCA analysis shown that Wuyi Rock Tea samples had significantly different alkaloid profile with green tea,Tieguanyin Tea and black tea.