食源性致病菌PCR检测前处理方法研究进展

摘要

以PCR(聚合酶链式反应)为代表的分子检测方法被广泛应用于食源性致病菌的快速检测,然而,在复杂的食品介质中实现微量致病菌的精确与快速检测,除了 PCR 体系构建之外,前处理方法也是快速检测的重要制约因素.文章对食源性致病菌 PCR 检测的前处理方法(包括离心、膜过滤、磁性分离等)进行了系统的综述,尤其较为深入地综述了磁性分离在PCR检测食源性致病菌前处理中的应用.%Molecular detection methods based on PCR (polymerase chain reaction)are widely used in the rapid detection of food-borne pathogens.However,in practical applications pretreatment method is also a critical step in rapid detection except the optimization of PCR.In this review,the pretreatment methods (including the cen-trifugation,membrane filtration,magnetic separation)for PCR de-tection of food-borne pathogens were systematically summeried.In particular,the magnetic separation was emphatically reviewed in the PCR detection of food-borne pathogens.