茶树紫芽中多种花色苷组分同时检测方法的建立

摘要

The purple bud tea zijuan,zixuan9803,Hongyafoshou an An73 shoots were used as research objects.Using three plant antho-cyanins as external standards,a HPLC method for simultanteous de-tection of various anthocyanins in purple bud tea tree was established.That is:after been extracted with 80% ethanol and re-fluxed,the tea is degreased with a volume ratio of 1︰1︰2 mixture of n-hexane:ethyl acetate:water.A C18column was used with aceto-nitrile and 0.2% phosphoric acid ultra-pure water as the mobile phase.Gradient,the organic phase elution gradient:0~55 min,10%~40%, flow rate 0.8 mL/min,column temperature 30 ℃,detection wave-length 520 nm.Each anthocyanins can be effectively separated,and the correlation coefficient of the standard curve is ≥0.9 9,the linear range is 5.00~75.00 μg/L.The precision and repeatability of the method is good.The relative standard deviation of the stability≤10%,the recovery rate is 75%~88%.Seven major anthocyanin com-ponents in four kinds of purple bud tea samples are effectively sepa-rated.The method has the characteristics of rapidity,sensitivity,ac-curacy,and low cost.It is also an analysis method which can be used to simultaneously detect and analyze various anthocyanin components in the purple bud tea.%以紫芽茶树品种(系)紫娟、自选9803、红芽佛手、安73新梢为研究对象,以3种植物花色苷为外标,建立同时检测紫芽茶树中多种花色苷的 HPLC分析方法.结果显示:茶叶经酸性80%乙醇回流提取后,以正己烷︰乙酸乙酯︰水体积比为1︰1︰2的混合溶剂脱脂,采用 C18色谱柱,以乙腈和0.2% 磷酸超纯水作流动相梯度洗脱,有机相洗脱梯度为:0~55 min、10%~40%,流速0.8 mL/min,柱温30 ℃,紫外检测波长520 nm,各花色苷组分可有效分离,标准曲线相关系数均≥0.99,线性范围为5.00~75.00 μg/L,该方法精密度、重复性良好,稳定性相对标准偏差值≤10%,加标回收率为75%~88%;4种紫芽茶树样品中的7种主要花色苷组分均得到有效分离.该方法具有快速、灵敏、准确、成本低的特点,同时是一种可用于同时检测分析紫芽茶树中多种花色苷组分的分析方法.