SNIF-NMR和IRMS技术在原产地葡萄酒鉴定中的应用

摘要

利用点特异性天然同位素分馏核磁共振技术（SNIF—NMR）和同位素比质谱仪（IRMS）技术测定葡萄酒中稳定性同位素D／H ^18O／^16O和^13C／^12C的比值，结果表明（D／H）Ⅱ、R、^18δ‰对产地的鉴别提供着主要信息，而（D／H）I与δ^13c‰提供了较次要的信息，单独使用SNIF—NMR结果只能区别开环境差异很大的地区，加上δ^13c‰后，可将温差大的地区区别开，但对环境相似的地区仍无法有效鉴别，最后将（D／H）Ⅱ、R、δ^13c‰、δ^18‰同时考虑，对于环境相似的地区也可以有效鉴别。研究结果为中国葡萄酒原产地鉴别提供了一种新的技术手段。%ABSTRACT SNIF-NMR and IRMS methods were used to determine the stable isotopic ratios of D / H and lSO/160 of wine. The results showed that methylene（D/H） Ⅱ of ethanol in wine , R andδ^18O‰ are the key determinants in i- dentifying the geographical origin of wines whereas methyl（D/H） I site andδ^13C‰ provide less significant information. Using the results of SNIF-NMR alone can only distinguish areas of considerably different environment. If coupled with δ^13C‰, the areas with different temperature can be distinguished, but the areas with similar environment still cannot be separated. If combined the values of （D/H）Ⅱ、R、δ^13c‰、δ^18‰, SNIF-NMR and IRMS methods can differentiate areas with similar environment. The research outcome provides a new technique to determine the geographical origins of Chinese wine.