类球红细菌产辅酶Q10发酵工艺的优化

摘要

类球红细菌(Rhodobacter sphaeroides)是一类高产辅酶Q10的光合细菌,该研究对R.sphaeroides EIM-8产辅酶Q1o的发酵条件和培养基进行了优化.采用单因素结合响应面设计优化后的培养基组成为:葡萄糖27.8 g/L、(NH4)2SO44.9 g/L、谷氨酸4.7 g/L、玉米浆粉2.5 g/L、MgSO49.5 g/L、FeSO41.5 g/L,NaC1 3.5 g/L,KH2PO44.0 g/L、辅液15.0 mL/L;通过单因素试验方法确定的培养条件为:pH 6.5、温度32℃、接种量10％、装液量40mL/250 mL,此条件下辅酶Q1o产量可达128.9 mg/L,比优化前提高了89.0％.%Rhodobacter sphaeroides is a kind of high yield coenzyme Q10 of photosynthetic bacteria.The medium composition and fermentation conditions for production of coenzyme Q10 by R.sphaeroides EIM-8 were optimized.Optimization of medium was performed by single factor and response surface design.The optimized medium composition was as follows: glucose 27.8 g/L, (NH4)2SO4 4.9 g/L, glutamic acid 4.7 g/L, corn steep liquor 2.5 g/L, MgSO4 9.5 g/L, FeSO4 1.5 g/L, NaCl 3.5 g/L, KH2PO4 4.0 g/L, auxiliary liquid 15.0 mL/L.The culture condition determined by single factor experiment was as follows; initial pH 6.5, temperature 32℃, inoculum 10% , medium volume 40 mL/250 mL.Using the described medium and condition, coenzyme Q10 yield could reach 128.9 mg/L which was 89.0% higher than results prior to optimization.