小分子糖及糖醇体外抑制α-葡萄糖苷酶活性的影响

摘要

In order to provide references for the development of hypoglycemic nutrition diet,the inhibitory effects of D-xylose,L-arabinose,trehalose,raffinose,lupeose,erythritol,isomaltitol,sorbitol,xylitol,and maltitol on the α-glucosidase activity were investigated.By using the high performance liquid chromatography method,the decomposition of sugars and sugar alcohols by α-glucosidase was detected,and their inhibitory effects on α-glucosidase were tested in the presence of sucrose.Further,the compounds with better inhibitory effects were designed to observe their interaction by using Calcusyn software.L-arabinose,D-xylose,trehalose,erythritol,isomaltitol and sorbitol all could inhibit the activity of α-glucosidase,and the value of IC50 of D-xylose,L-arabinose,trehalose,erythritol,isomaltitol and sorbitol were 23.89 mg/mL,15.03 mg/mL,9.75 mg/mL,3.19 mg/mL,20.64 mg/mL,106.19 mg/mL,respectively.The inhibition of α-glucosidase were significantly increased when erythritol was combined with trehalose or isomaltitol (Combination Index ＜ 1) and showed a synergistic effect.The compounds of erythritol and trehalose or isomaltitol may contribute to the enhancement of inhibition of α-glucosidase.%研究了D-木糖、L-阿拉伯糖、海藻糖、棉籽糖、水苏糖、赤藓糖醇、异麦芽酮糖醇、山梨糖醇、木糖醇以及麦芽糖醇等小分子糖及糖醇对α-葡萄糖苷酶活性的抑制作用,以期为降糖营养食品的开发提供参考.通过高效液相色谱法,确定α-葡萄糖苷酶对糖及糖醇的分解作用,进一步检测各种糖及糖醇在蔗糖底物存在下对α-葡萄糖苷酶的抑制作用,将抑制效果较好的糖及糖醇进行复配,利用Calcusyn软件计算复配结果,观察样品的相互作用.D-木糖、L-阿拉伯糖、海藻糖、赤藓糖醇、异麦芽酮糖醇、山梨糖醇均能抑制α-葡萄糖苷酶的活性,且D-木糖、L-阿拉伯糖、海藻糖、赤藓糖醇、异麦芽酮糖醇以及山梨糖醇的IC50值分别为23.89 mg/mL、15.03 mg/mL、9.75 mg/mL、3.19 mg/mL、20.64 mg/mL、106.19 mg/mL.赤藓糖醇分别与海藻糖、异麦芽酮糖醇复配后对α-葡萄糖苷酶的抑制作用明显增强(联合指数CI＜1),表现出协同效应.结论:赤藓糖醇与海藻糖或异麦芽酮糖醇复配后(CI＜1)有助于增强对α-葡萄糖苷酶的抑制作用.