菟丝子醇提液对小鼠脑缺血再灌注损伤的保护作用

摘要

Objective To study the improvement of alcoholic extraction of cuscutae semen (AECS) on learning and memory impairment induced by cerebral ischemia- reperfusion (I/R) in mice, as well as its effect and mechanism on the the activities of relative enzymes in the liver and brain tissues. Methods AECS was obtained by ultrasonic extraction. Cerebral I/R injury was produced in conscious mice by temporarily obstructing bilateral common carotid arteries. The protective effects of three different doses of AECS on learning and memory impairment in I/R mice were observed using the passive avoidance test and biochemical methods of observing the glutathione peroxidase (GSH-Px) activities in liver and brain. Results The medium and high doses of AECS could decrease the number of error and prolong the latent time in passive avoidance test and increase the activity of GSH-Px in brain and liver tissues. Conclusion AECS shows protective effects on learning and memory impairment of mice induced by cerebral I/R injury. The possible mechanism was argued that AECS can increase the activity of GSH-Px in brain and liver tissues.%　　目的探讨菟丝子醇提液（AECS）对脑缺血再灌注（I/R）小鼠学习记忆障碍的改善作用，对肝脑组织中相关酶的作用及其作用机制。方法利用超声提取法制备AECS；采用暂时性阻断两侧颈总动脉的方法制备小鼠脑I/R损伤的模型，进行避暗实验，观察AECS对小鼠学习记忆功能的保护作用；生化方法测定肝脑组织中谷胱甘肽过氧化物酶（GSH-Px）活性。结果AECS中、高剂量可明显减少脑I/R小鼠避暗实验中错误次数，延长潜伏期；AECS中、高剂量能不同程度地提高肝、脑组织中GSH-Px活性。结论AECS对脑I/R所致小鼠记忆功能障碍具有保护作用，其作用机制可能为增强肝、脑组织中GSH-Px活性。