两种方法检测替加环素对产碳青霉烯酶肺炎克雷伯菌体外药敏结果的分析

摘要

Objective To study the difference of two testing methods on the susceptibility results of tigecycline against car-bapenemase-producing Klebsiella pneumoniae. Methods The susceptibility of 23 strains of carbapenemase-producing Klebsiella pneumoniae to tigecycline were detected with Disc diffusion and MIC Test Strip (MTS), and the results of different methods were compared. Results The susceptibility rates of 23 straints of carbapenemase-producing Klebsiella pneumoniae were 95.7% and 100% respectively for disc diffusion and MTS. If MTS was taken as the reference methods, the minor error rate of disc diffusion method was 4.3%. Conclusion There is difference on susceptible rate between the two methods for the susceptibilty testing of tigecycline against carbapenemase-producing Klebsiella pneumoniae.%目的：比较两种方法检测替加环素对产碳青霉烯酶肺炎克雷伯菌体外药敏结果的差异。方法分别采用纸片扩散法和MIC测试条(MTS)法检测替加环素对23株产碳青霉烯酶肺炎克雷伯菌的体外敏感性。结果 MTS法敏感率为100%；而纸片扩散法敏感率为95.7%，出现4.3%的中介菌株。若以MTS法为标准，纸片扩散法检测的次要误差率为4.3%，无重要误差和严重误差。结论纸片扩散法和MTS法检测替加环素对产碳青霉烯酶肺炎克雷伯菌的体外敏感性时，其结果存在差异。