Method and apparatus for phenotypic detection of carbapenemase and carbapenemase formers

摘要

The invention provides a method and apparatus for phenotypic detection of carbapenemases and carbapenemase formers by using a substrate of the general formula to a sample suspected of containing such carbapenemase formers and / or carbepenemases A- (L) -M ₁ - (X) -Z wherein M _{1 is} a carbapenem backbone, one of A or Z is a quencher, the other of A or Z is a fluorophore, L is an optional linker, X is an optional leaving group for linking Z to the carbapenem backbone, and Z is an optional leaving group becomes. The invention further provides a method for the phenotypic detection of resistant bacteria, in particular 3 MRGN or 4MRGN, by liberating the enzymes of a bacterial culture into a lysate during lysis and subsequently subjecting the lysate as the sample to be examined to an abovementioned method in order to obtain the lysate Presence of resistance-conferring carbapenemases phenotypically detected.