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Primers for detection and typing of producing bacterial strains carbapenemases, method and detection kit

机译:用于检测和鉴定生产性细菌碳青霉菌的引物,方法和检测试剂盒

摘要

pairs of primers, their use for gene detection and carbapenemases carbapenemases producing bacterial strains, and a diagnostic method using said primer set are proposed. the use of a group of probes within each amplification reaction, more preferably multiplex PCR for selective detection and identification of genes carbapenemases from a biological sample is further proposed preferably. Primers object of this invention can be used for the preparation of a kit for identification and detection of genes carbapenemases, preferably carbapenemases genes contained in bacterial strains producing said enzyme.
机译:提出了两对引物,它们用于基因检测和碳青霉烯酶产生碳青霉菌酶的细菌菌株,以及使用所述引物组的诊断方法。优选进一步建议在每个扩增反应中使用一组探针,更优选在多重PCR中从生物样品中选择性检测和鉴定碳青霉烯酶的基因。本发明的引物可以用于制备用于鉴定和检测碳青霉酶的基因的试剂盒,所述基因最好是包含在产生所述酶的细菌菌株中的碳青霉酶的基因。

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