目的：探讨卵巢早衰大鼠卵巢中原癌基因c-erbB2和EGFR的表达。方法20只有动情周期的雌性大鼠随机分为空白组和实验组。实验组大鼠口服雷公藤多甙片悬浮液,按照50mg/Kg.d，共14d,造POF模型；空白组大鼠同剂量生理盐水灌胃，1次/d，共14d。给药结束后24h,空白组和实验组大鼠取血标本后处死，留取卵巢。留取的卵巢制切片后行HE染色和免疫组化。结果卵巢HE染色发现空白组卵泡生长活跃,可见较多的成熟和次级卵泡,而实验组卵巢次级和成熟卵泡明显减少(P<0.01)，可见较多闭锁卵泡(P<0.01)，卵泡的颗粒细胞层次明显减少；免疫组化发现空白组ErbB2蛋白和EGFR蛋白阳性信号明显，主要位于卵母细胞胞膜上，为深棕色染色颗粒，实验组则阳性表达较少。结论 c-erbB2基因和EGFR可能参与了卵巢早衰中卵泡成熟障碍及卵泡凋亡的调控。%Objective To explore the expressions of proto-oncogene c-erbB2 and EGFR in rats with premature ovarian fail-ure. Methods Twenty female estrus rats were randomly divided into blank group and experimental group. The rats in experimen-tal group were treated with pouring Tripterygium wilfordii polyglycoside (TWP) suspension liquid (50mg/kg·d) into stomach for 14 days;while the rats in blank group were treated with pouring normal saline of the same volume into stomach for 14 days. After the models were made, ovarian sections in the two groups were taken to assess the expression of proto-oncogene c-erbB2 and EGFR in ovarians and to observe the ovarian histomorphological changes. Results The numbers of mature and secondary follicles in ex-perimental groups were significantly reduced(P<0.01),whereas the numbers of atresia follicles were markedly increased as compared with that of the controls (P<0.01). The expression of ErbB2 and EGFR protein in the blank group was mainly on the cell membrane of oocytes, but their expression levels in the experimental group were much lower than that in different stages of the follicles. Conclusion EGFR and c-erbB2 might be involved in the maturation and apoptosis of ovarian follicles.
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