CD4+CD25+Treg细胞及其相关细胞因子在HBV感染孕妇胎盘组织中的表达

摘要

目的：研究胎盘组织中CD4+CD25+Treg 细胞及其相关因子在 HBV 感染孕妇胎盘组织中的表达，探讨孕产妇HBeAg阳性时宫内感染率高的机制。方法采用免疫组化(二步法)检测HBV感染孕妇胎盘组织中Foxp3、IL-10及TGF-β1的表达水平。结果 Foxp3、TGF-β1及IL-10阳性细胞都主要定位于胎盘绒毛滋养细胞、绒毛血管内皮细胞、蜕膜细胞，在绒毛间质细胞有少量表达，表达部位在细胞浆；宫内感染组胎盘组织中Foxp3、IL-10、TGF-β1较宫内未感染组阳性表达率均显著增高(P<0.05)；HBeAg阳性组胎盘组织中Foxp3、IL-10、TGF-β1较HBeAg阴性组阳性表达率均显著增高(P<0.05)。结论胎盘组织中CD4+CD25+Treg细胞的增多可能是发生HBV宫内感染的危险因素之一；孕妇HBeAg阳性会增加HBV宫内感染发生率；HBeAg阳性孕妇胎盘组织中存在Tregs增多及其相关细胞因子表达水平升高，这可能是HBeAg阳性孕妇HBV宫内感染发生率高的机制之一。%Objective To investigate the expression of Foxp3, IL-10 and TGF-β1 in the placenta and its relationship with HBV intrauterine infection. Methods Immunohistochemistry (IHC) was utilized to identify the location of Foxp3, IL-10 and TGFβ1. Maternal and neonatal HBV infection were detected by ELISA. Results The expression of Foxp3, IL-10 and TGF-β1 mainly located in placental trophoblast cells, decidual cells and villous capillary endothelial cells, with a small amount of expres-sion in villous stromal cells. The positive rates of Foxp3, IL-10 and TGF-β1 in intrauterine infected group were significantly high-er than those in intrauterine uninfected group(P<0.05). The positive rates of Foxp3, IL-10, TGF-β1 in HBeAg positive group were significantly higher than those in HBeAg negative group (P<0.05). Conclusion The high expression of Foxp3, IL-10 and TGF-β1 in placenta may be related to HBV intrauterine infection, as Foxp3 is a specific marker of CD4+CD25+Tregs, which also indicates the up-regulated CD4+CD25+Treg cells might play a role in HBV intrauterine infection.