乌司他丁对大鼠脑缺血再灌注后脑组织紧密连接蛋白1及血清神经元特异性烯醇化酶表达的影响Δ

摘要

目的：探讨乌司他丁对缺血再灌注脑损伤的保护作用。方法：选取健康雄性SD大鼠72只，将所有大鼠以随机数字表法分为假手术组、缺血再灌注损伤模型组和乌司他丁处理组，每组各24只。建立大鼠缺血再灌注模型，检测3组大鼠脑组织紧密连接蛋白1（zonula occluden－1，ZO－1）及血清神经元特异性烯醇化酶（NSE）的表达变化。结果：与缺血再灌注损伤模型组大鼠相比，乌司他丁处理组大鼠脑组织中ZO－1蛋白表达显著增加，NSE表达明显减少，差异均有统计学意义（P＜0．05）。结论：乌司他丁可通过抑制脑缺血再灌注后血－脑脊液屏障通透性的增加、减少NSE表达而发挥脑保护作用，其保护血－脑脊液屏障的机制可能与增加ZO－1蛋白的表达有关。%OBJECTIVE:To probe into the protective effects of ulinastatin on ischemia-reperfusion injury. METHODS:72 healthy male SD rats were randomly selected to be divided into sham operation group, schemia-reperfusion injury model group and ulinastatin group, with 24 cases in each.Schemia-reperfusion injury model was established, expression changes of zonula occluden-1(ZO-1) and neurone specific enolase(NSE) in brain tissues of three groups were detected.RESULTS: Compared with schemia-reperfusion injury model group, expression of ZO-1 protein in ulinastatin group significantly increased, while NSE decreased significantly, with statistically significant difference(P<0.05).CONCLUSIONS:Ulinastatin can play a protective role in the brainm by inhibiting the increased cerebral ischemia and reperfusion brain barrier permeability, and reducing expression of NSE;and its protective blood-brain barrier may be related to upregulation expression of ZO-1 protein.