The role of protein kinase B (PKB) in the regulation of early development of fertilized eggs in mouse and its biological function on the development of mouse 1|cell stage fertilized eggs were studied.The kinase activity of PKB was detected,and mRNA and protein level of PKB both in MⅡ oocytes and 1|cell stage fertilized eggs were semi|quantitatively assayed by Western blotting and RT|PCR.10 μmol/L LY294002,the inhibitor of PKB,was microinjected into 1|cell stage fertilized eggs of mouse to observe its effect on the development of mouse 1|cell stage fertilized eggs.The results showed that the activity of PKB increased obviously after the fertilization,the mRNA and protein level of PKB also increased obviously in 1|cell stage fertilized eggs,while in MⅡ oocytes the mRNA level was not detected.The protein level was lower than that in the 1|cell stage fertilized eggs.After microinjecting LY294002 the development from 1|cell stage to 2|cell stage was inhibited obviously.All the results demonstrate that PKB exists in the fertilized eggs,and the activity,expression and transcription level increase after fertilization.PKB may play a positive role during the early development of fertilized eggs in mouse from 1|cell to 2|cell stage,but the mechanism is still unknown.These experiments are helpful for the further study of PKB on the regulation of early development of fertilized eggs.
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