Activity of 1,1,1- and 1,1,3-Trichloroacetones in a Chromosomal Aberration Assay in CHO (Chinese Hamster Ovary) Cells and the Micronucleus and Spermhead Abnormality Assays in Mice

摘要

1,1,1- and 1,1,3-trichloroacetones (TCA) result from the disinfection of municipal water supplies with chlorine and are direct-acting mutagens in the Ames/Salmonella assay. The study further investigated the genotoxicity of these compounds in mammalian cells using an in vitro chromosomal aberration assay in Chinese hamster ovary (CHO) cells and the micronucleus and spermhead abnormality assays in mice. Both compounds induced significant increases in structural chromosomal aberrations in CHO cells in the presence and in the absence of rat S9 metabolic activation (MA). 1,1,3-TCA was more cytotoxic to CHO cells but 1,1,1-TCA resulted in a higher proportion of cells with aberrations. The clastogenic activities of both compounds were reduced in assays conducted with MA. Results indicate that the drinking water contaminants 1,1,1- and 1,1,3-TCA are clastogenic in vitro, but are not clastogenic to bone marrow cells in vivo and do not adversely affect several indicators of testicular function in mice. (Copyright (c) 1988 Elsevier Science Publishers B.V. (Biomedical Division).)