目的：观察处于牙周炎发生不同时期的牙龈组织的中CD4+CD25+Treg比例变化，探讨CD4+CD25+Treg在牙周炎发生时的免疫调节作用。方法选择35只成年SPF级Wistar大鼠，丝线结扎加高糖饮食建立大鼠牙周炎发生的动物模型，获得类似牙周炎自然病程的连续组织标本，病理切片确定病损，分别获取第2、4、8、12周的大鼠牙龈组织，术前未结扎组对照。以流式细胞仪检测不同时段牙周病损和外周血中CD4+CD25+Treg的比例变化。结果建立了Wistar大鼠牙周炎发生的动物模型，病理切片确定了牙周病损的存在。结果显示伴随牙周炎发生的牙槽骨的吸收逐渐增多。流式细胞仪结果显示结扎后第2、4、8、12周牙龈中CD4+CD25+Treg占CD4+T细胞的比例为9.11%、13.32%、15.16%，11.06%。与术前存在显著性差异。结论牙周炎发生时牙龈病变中CD4+CD25+Treg自逐渐升高，稳定期回落，牙周炎发生发展规律一致。从而证实CD4+CD25+Treg细胞参与介导了牙周炎发生的免疫调控%Objective CD4+CD25+ regulatory T (Tr) cells are critical in regulating the immune response. The aim of this study was to identify CD4+CD25+ T cells in periodontal lesion tissues during the progression of experimental periodontitis. Methods The frequency of CD4+CD25+T cells in periodontal lesion tissues during the progression of experimental periodontitis was measured by fluorescence-activated cell sorter(FACS) me thod. Results During the progression of experimental periodontitis, the percentage of CD4+CD25+ Treg increased in periodontal lesion tissues. Conclusion CD4+CD25+ Tr cells may play regulatory roles in the progression of experimental periodontitis.
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