目的：研究错配修复基因家族成员hMLH1、hMSH2、hMSH6在结直肠癌中的表达及其临床意义。方法采用免疫组化SP法检测72例结直肠癌组织中hMLH1、hMSH2、hMSH6蛋白的表达,并分析三者的相关性及与临床病理特征的关系。结果72例结直肠癌组织hMLH1、hMSH2、hMSH6表达缺失率分别为73．6%、65．3%和43．1%。hMLH1、hMSH2表达缺失率与肿瘤直径、分化程度、Duke's分期、淋巴结转移及远处转移有关（P0．05)；hMSH6蛋白表达缺失率与肿瘤分化程度、Duke's分期、淋巴结转移有关（P0．05)。结直肠癌hMLH1、hMSH2蛋白表达缺失率呈正相关（r=0．295, P0．05)。结论 hMLH1、hMSH2、hMSH6可能在结直肠癌的发生、发展中起重要作用,联合检测对结直肠癌的诊断与治疗有一定价值。%Objective To study the mismatch repair gene family members' hMLH1, hMSH2, hMSH6 expression and the relationship between the three in colon-rectal cancer and its clinical significance. Methods Immunohistochemical SP method was used to detected 72 cases of colon-rectal cancer for tissues hMLH1, hMSH2, hMSH6 protein expression, and a-nalysis of the correlation and pathological features was made. Results For hMLH1, hMSH2, hMSH6 expression in 72 cases of colon-rectal cancer, tissues deletion rate was 73. 6%, 65. 3 and 43. 1%, respectively. In colon-rectal cancer and hMLH1, hMSH2 expression missing rate and protein expression were correlated to tumor size, differentiation, Dukes stage, lymph node metastasis and distant metastasis (P0. 05). hMSH6 protein deletion rate was related with degree of tumor differentiation, Dukes stage, lymph node metastasis ( P 0. 05). hMLH1, hMSH2 protein expression was positively correlated with expression missing in colon-rectal cancer in the (r= 0. 295, P0. 05). Conclusion hMLH1, hMSH2, hMSH6 may play an important role in the occurrence of colon-rectal cancer, and the combined detection of colon-rectal cancer may have some reference value in diagno-sis and treatment of colon-rectal cancer.
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