Objective: The present study aimed to examine the expression of mismatch repair genes, namely, human mutL homo-log 1(hMLH1) and human mutL homolog 2 (hMSH2), in colorectal carcinoma, and to investigate the relationship of hMLH1 and hMSH2 expression with clinicopathological features. Methods: Seventy-two patients with pathologically colorectal carcinoma and un-derwent surgical resection were included in the study. All patients did not undergo preoperative chemotherapy or radiotherapy. Immuno-histochemical method was used to examine the hMLH1 and hMSH2 expressions in the colorectal carcinoma. The correlation of hMLH1 and hMSH2 expression with pathological features was investigated. Results: The loss rate of hMSH2 expression in colorectal cancer tissue was higher than that in normal tissue (88.9% vs. 28.0%, χ 2=37.622, P<0.001). The loss rate of hMSH2 expression in-creased with T staging, but without significant difference. The loss rate of hMSH2 expression in patients with lymph node metastases was 97.6%, whereas the rate was 77.4% in patients with no lymph node metastases. Moreover, the results were significant (χ2=7.251, P=0.007). The loss rate of hMLH1 expression in colorectal cancer tissue was 90.3% (65/72). The loss rate of hMLH1 expression was not correlated with tumor site, tumor stage, and tumor grade. Conclusion: The combined detection of hMLH-1 and hMSH-2 expressions may be used to evaluate the prognosis of colorectal cancer.% 目的：评价hMLH1和hMSH2蛋白在结直肠癌中的表达及意义。方法：选取2009年1月至2011年12月经病理学确诊的结肠癌手术切除标本72例，所有患者在术前均未接受过放疗或化疗，内镜活检取正常肠黏膜上皮25例。免疫组织化学检测hMLH1和hMSH2蛋白表达情况。结果：结直肠癌组织中hMSH2缺失率为88.9%（64/72），高于正常肠组织中hMSH2蛋白缺失率28.0%（7/25）。hMSH2蛋白缺失率随T分期增加而增加，但差异无统计学意义（χ2=37.622，P<0.001）；hMSH2蛋白缺失率与N分期相关，有淋巴结转移者的 hMSH2蛋白缺失40例，缺失率达97.6%（40/41），无淋巴结转移患者的 hMSH2蛋白缺失率为77.4%（24/31，χ2=7.251，P=0.007）。而 hMLH1蛋白缺失率为90.3%（65/72），高于正常组肠组织中 hMLH1蛋白缺失率为32.0%（8/25，χ2=33.847，P<0.001），但与肿瘤部位、分期、分化程度均无关。结论：在结直肠癌组织中存在错配修复基因hMSH2和hMLH1蛋白缺失，且表达缺失与肿瘤分期有关。通过免疫组织化学方法检测hMLH1和hMSH2蛋白表达可以简便、准确地发现错配修复基因的突变，从而对其后期的治疗和预后判断有参考价值。
展开▼
