多西环素对哮喘大鼠血清 IgE、肺组织磷酸化 p38及基质金属蛋白酶9的影响

摘要

Objective To observe the influence of doxycycline on airway inflammation and remodeling in asthmatic rats.Methods Thirty-three SD male rats were randomly divided into three groups (1 1 rats in each group):normal control group,asthma group and doxycycline intervention group.After inhalation of doxycycline,the change of matrix metalloproteinase 9(MMP-9),phosphorylation of p38 of lung tissue and serum IgE were investigated.Results MMP-9 in lung tissue was significantly higher in asthma group and intervention group than in control group.The level of serum IgE was significantly higher in asthma group than in intervention group.The expression of phosphorylated p38 in asthma group was significantly higher than that of intervention group.Conclusion Doxycycline can alleviate inflammation of airway through reducing inflammatory cells and improve airway remodeling through downregulating the expression of MMP-9.In addition,doxycycline can ameliorate airway inflammation,airway remodeling and airway hyper-responsiveness by influencing serum IgE and the protein expression of phosphorylated p38 mitogen-activated protein kinase in lung tissue.%目的：建立大鼠哮喘模型，探讨多西环素对哮喘大鼠气道炎症和气道重塑的影响。方法取健康雄性大鼠33只，随机分为3组：正常对照组、哮喘模型组、多西环素干预组。应用给予哮喘大鼠雾化吸入多西环素，观察干预后大鼠肺组织中基质金属蛋白酶9(MMP-9)及磷酸化的 p38、血清中 IgE 水平的变化。结果与正常对照组相比，哮喘模型组、多西环素干预组 MMP-9的水平显著升高(P <0.05)。哮喘模型组血清 IgE、肺组织磷酸化的 p38磷酸化的 p38/β-actin 水平明显高于多西环素干预组(P <0.01)。结论多西环素可减少肺泡灌洗液中炎性细胞的数量，从而减轻气道炎症；多西环素可下调 MMP-9的水平，从而减缓气道重塑。通过对血清 IgE 和肺组织磷酸化的 p38的影响，多西环素减缓哮喘的气道炎症、气道重塑和气道高反应。