十二烷基硫酸钠对多重耐药大肠埃希菌Ⅰ类整合子作用的初步探讨

摘要

Objective To discuss the effect of SDS on the class Ⅰ integron of multidrug resistant Fscherichia coji(E.coli).Methods The susceptibilities of 71 E.coli strains to 4 antibiotics were detected by disk diffusion test.Genes localing at 3 different places in class Ⅰ integron were detected by PCR.The class Ⅰ integronpositive E.coli strains, which were multidrug resistant,were treated with SDS,then detected by PCR and MIC values.Results There were 23(32.39％)multidrug resistant strains in 71E.coli strains. Class Ⅰ integron-positive rate of 71 E.coli strains was 88.73％.The detection rates of IntI1 genes,genetic markers genes and variable region genes were respectively 74.65％ ,78.87％ and 21.13％. The class Ⅰ integron-positive rate and gentamycin and cefotaxim MIC values of multidrug resistant E.coli strains decreased statistically with SDS.Conclusion SDS has some effect on the integron calss Ⅰ of multidrug resistant E.coli.%目的 探讨十二烷基硫酸钠(SDS)对多重耐药大肠埃希菌(E.coli)Ⅰ类整合子的作用.方法 采用纸片扩散法(K-B法)测定71株大肠埃希菌对3类4种常用抗菌药物的药敏情况,聚合酶链反应(PCR)检测Ⅰ类整合子3个不同部位的基因.对经SDS处理的Ⅰ类整合子阳性多重耐药大肠埃希菌的Ⅰ类整合子基因与最低抑菌浓度(MIC)值进行测定与比较.结果 71株大肠埃希菌的多重耐药率为32.39%(23株),Ⅰ类整合子的总检出率为88.73%,不同部位基因检出率分别为:整合酶基因74.65%、遗传标记基因78.87%、可变区基因21.13%.SDS处理前、后多重耐药大肠埃希菌中的Ⅰ类整合子基因阳性率差异有统计学意义(P＜0.05);对庆大霉素、头孢噻肟的MIC值差异均有统计学意义(P＜0.05).结论 SDS对多重耐药大肠埃希菌的Ⅰ类整合子有一定的影响.