不同程度宫颈病变单一、多重高危型 HPV感染状态下E6／E7 mRNA的表达

摘要

Objective To investigate the HPV genotype distribution characteristics in the patients with different degrees of cer-vical diseases and the expression of high-risk HPV E6/E7 mRNA in the patients with single or multiple infections and its influence on the HPV pathogenicity.Methods A total of 236 formalin-fixed,paraffin-embedded specimens were collected from women with different degrees of cervical diseases.The gene chip technique was adopted to conduct the genotype detection.The in 23 kinds of HPV genotype on 236 specimens.The TaqmanRT-PCR technique was adopted to measure the expression of high-risk HPV E6/E7 mRNA.Results The HPV positive rate was 65.3%(154/236),including 233 cases of HPV subgenotye infection(single or multi-ple),the expression of HPV E6/E7 mRNA was detected in 60.9% of cases(142/233),the HPV positive rate and the expression level of HPV E6/E7 mRNA were increased with the increase of the cervical lesion grade(P<0.05).In the high-risk infection cases of lesion≥CINⅡ,the expression rate of HPV45 E6/E7 mRNA was highest(93.3%),followed by HPV16(81.0%)and HPV18 (78.4%).In the lesions ≥CINⅡ,the expression of E6/E7 mRNA was detected in 93.7% of cases(118/126),while which in le-sions < CINⅡwas detected in only 39.3 % of cases(11/28).In different degrees of cervical lesions,the expression rate of E6/E7 mRNA in the cases of multiple infection was higher than that in the caes of single infection(61.2%vs.38.8%,P<0.05).Conclu-sion In any degree of cervical lesion,HPV multiple infection,especially high expression level of HPV45 E6/E7 mRNA could in-crease the HPV pathogenicity.%目的：探讨不同程度宫颈病变的 HPV基因型分布特点及高危型 HPV E6/E7 mRNA在单一、多重感染病例中的表达，以及其对 HPV致病力的影响。方法收集不同程度宫颈病变石蜡组织标本共236份。采用基因芯片技术对236份标本进行23种 HPV基因型分型检测，并采用Taqman逆转录-PCR技术测定高危型 HPV E6/E7 mRNA的表达。结果 HPV阳性率为65．3％（154/236），其中包含了233个 HPV亚型（单一或多重）感染，60．9％（142/233）病例可检测出 E6/E7 mRNA的表达，HPV阳性率和 E6/E7 mRNA表达水平随宫颈病变级别的上升而升高（P＜0．05）。在大于或等于 CINⅡ的高危亚型感染病例中， HPV45的 E6/E7 mRNA表达率最高（93．3％），其次为 HPV16（81．0％）、HPV18（78．4％）。在大于或等于 CINⅡ的病变中，93．7％（118/126）病例可检测到 E6/E7 mRNA的表达，而小于CINⅡ的病变中仅有39．3％（11/28）的病例可检测到 E6/E7 mR-NA的表达。在所有不同程度宫颈病变中，多重感染病例E6/E7 mRNA表达率较单一感染病例高（61．2％vs．38．8％，P＜0．05）。结论在任何程度的宫颈病变中，HPV多重感染，尤其 HPV45的 E6/E7 mRNA高表达水平可能会提高 HPV的致病力。