DHA 对 IFN-γ诱导的3T3L1脂肪细胞 MHC Ⅱ及炎症分子表达的影响

摘要

Objective To investigate the effects of DHA on expression of IFN-γinduced M HCⅡ and inflammatory cytokine in 3T3L1 adipocytes .Methods Stimulating the fully differential 3T3L1 cells with IFN-γ ,we compared theexpression of M HC Ⅱgenes and inflammatory cytokine in the presence or absence of DHA by real-time PCR and Western blot .Results IFN-γ-mediated induction increased the expressions of Ciita ,H2Ab1 ,iNOS ,MCP-1 ,IL-6 in 3T3L1 adipocytes .In the presence of DHA ,the expres-sions of iNOS ,IL-6 and Ciita ,H2Ab1 were down-regulated .Conclusion These results demonstrate that exposure to DHA reduced the level of the expressions of M HC Ⅱ and inflammatory cytokine inmature 3T3L1 adipocytes and led to a significant reduction in in-flammasome .%目的：探讨二十二碳六烯酸（DHA）对 IFN-γ诱导的3T3L1脂肪细胞组织相容性复合体Ⅱ（M HC Ⅱ）及炎症分子MCP-1、IL-6、iNOS 表达的影响。方法3T3L1脂肪细胞诱导分化成成熟脂肪细胞，加入 IFN-γ诱导炎症反应，再加入 DHA 处理，通过 real time-PCR 及 Western blot 观察 DHA 对分化成熟的脂肪细胞 M HC Ⅱ及炎症分子 MCP-1、IL-6、iNOS 表达的影响。结果分化成熟的脂肪细胞加入 IFN-γ后，M HC Ⅱ的相关基因 Ciita 及 H2Ab1，炎症分子 MCP-1、IL-6、iNOS 明显上升，加入DHA 后，Ciita 、H2Ab1、iNOS 、IL-6表达明显下调，MCP-1的变化比较差异无统计学意义（P ＞0．05）。结论 DHA 能明显下调IFN-γ诱导的3T3L1成熟脂肪细胞 M HC Ⅱ及炎症分子表达，DHA 通过下调 M HC Ⅱ基因改善脂肪细胞的炎症。