Objective To explore the effect of araloside on the proliferation and apoptosis of HeLa cells.Methods Human cervical cancer cell line HeLa was cultured in vitro,the experiment was divided into 4 groups as follows:blank group,araloside trea-ted groups(50,100,200 μg/mL).Normal saline and araloside (50,100,200 μg/mL)were gave,respectively.24,48 and 72 hours lat-er,the cells were collected.Cell proliferation was detected by MTT,cell apoptosis was determined by flow cytometry,the expression of pAkt1,pIкBα,NF-κB (p65),Bcl-2 and Caspase-3 were evaluated by western blot.Results Araloside obviously inhibited the pro-liferation and increased the apoptosis level of HeLa cells in a time-dose dependent manner.Moreover,Araloside significantly de-creased the phosphorylation of Akt1 and IκBα,reduced the expression of NF-κB(p65)and Bcl-2,whereas obviously increased Caspase-3 content.Conclusion Araloside could inhibit the proliferation and promote the apoptosis of HeLa cells via suppressing Akt/NF-кB signaling pathway.%目的:探讨!木皂苷对人宫颈癌 HeLa 细胞增殖和凋亡的影响。方法以体外培养的人宫颈癌 HeLa 细胞为研究对象,实验分为阴性对照组和!木皂苷组(50、100、200μg/mL)共4组,分别给予 RPMI-1640培养液和!木皂苷(50、100、200μg/mL)处理。24、48和72 h 后,分别收集各组细胞,采用 MTT 法检测细胞的增殖情况,流式细胞仪检测细胞凋亡,Western blot 技术测定细胞内蛋白(pAkt1、pIкBα、NF-κB p65、Bcl-2和 Caspase-3)表达水平。结果!木皂苷呈时间-剂量依赖性地抑制 HeLa 细胞的增殖,并增加其凋亡水平。另外,!木皂苷显著降低 Akt1和 IкBα的磷酸化水平,减少 NF-κB(亚基 p65)和 Bcl-2的蛋白表达,相反却显著增加 Caspase-3的含量。结论!木皂苷通过降低 Akt/NF-κB 信号通路的活性抑制宫颈癌 HeLa 细胞的增殖并促进其凋亡。
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