Objective To investigate the changes of CD35 and CD59 of erythrocyte and T lymphocyte subsets and their sig-nificances in patients with bone and joint tuberculosis and pulmonary tuberculosis .Methods Venous blood was extracted from 60 bone and joint tuberculosis patients ,60 pulmonary tuberculosis patients ,and 90 healthy individuals(control group) .Flow cytometry (FCM ) was used to detect the concentration of T lymphocyte subsets ,such as CD3 + ,CD4 + ,CD8 + and CD4 + /CD8 + ,as well as im-mune molecules of erythrocyte ,such as CD35 and CD59 .Results CD3 + ,CD4 + ,CD4 + /CD8 + ,CD35 and CD59 in bone and joint tu-berculosis group and pulmonary tuberculosis group were significantly lower than those in control group(P< 0 .05) ,while CD8 + was higher than in control group(P< 0 .05) .Furthermore ,there was a positive correlations between CD35 and the percentage of CD3 +and CD4 + in the bone and joint tuberculosis group and pulmonary tuberculosis group(P< 0 .05) ,while CD35 had no obvious corre-lation with CD8 + and CD4 + /CD8 + (P> 0 .05) .Conclusion Compared with the ordinary people ,there are significant differences be-tween erythrocyte and T lymphocyte immune in patients with bone and joint tuberculosis and pulmonary tuberculosis ,but it is the reason or the results need to be further studied .%目的：了解骨关节结核与肺结核患者外周血 T 淋巴细胞亚群和红细胞 CD35、CD59的变化及其意义。方法收集2013年10月至2015年4月收治的60例骨关节结核患者，60例肺结核患者，选择同期健康体检者90人作为对照组。采用流式细胞仪抗体双标法检测外周静脉血 T 淋巴细胞亚群（CD3＋、CD4＋、CD8＋和 CD4＋／CD8＋）和红细胞 CD35、CD59的表达。结果与对照组比较，骨关节结核组和肺结核组 CD3＋、CD4＋和 CD4＋／CD8＋显著降低（P＜0．05），CD8＋明显增高（P ＜0．05）；红细胞CD35、CD59表达显著降低（P ＜0．05）。骨关节结核组与肺结核患组 CD35与 CD3＋、CD4＋呈正相关（P ＜0．05），而与 CD8＋、CD4＋／CD8＋无明显相关性（P＞0．05）。结论骨关节结核与肺结核患者 T 细胞免疫和红细胞免疫与常人比较均存在显著差异，但其是原因还是结果尚需进一步研究。
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