为了探究青霉菌灭活菌丝体（DMP）在烟草抗性中的作用机制，以DMP水溶液处理的烟草红花大金元叶片为材料，利用抑制消减杂交（SSH）技术构建了cDNA文库，并对文库进行了分析。SSH获得356个克隆，挑选102个克隆，测序成功77个。Blastn比对出47个不同功能的基因。Blastx比对出26个不同功能的蛋白；7种未知功能的预测蛋白；2个克隆没有比对出相关蛋白。所有克隆抗病防御、能量代谢和细胞保护相关基因较多，分别占总数的40.3%、23.4%和14.9%。其余有关细胞结构、蛋白合成、转录及信号传导的基因相对较少，分别占到8.5%、4.3%、4.3%和4.3%。%Dry mycelium of Penicillium chrysogenum (DMP) could protect plant from disease. But its mechanism of induction and signaling pathways was unclear. Analyzing the genes expression in the plant treated by DMP was a good way to find out how inducing agent works. SSH technology was used to establish a cDNA library of tobacco induced by Dry mycelium of Penicillium chrysogenum. In the library 356 positive clones were obtained, of which 102 clones were selected for sequencing and 77 were sequenced successful. The result of Blastn showed that 47 genes’ functions were obtained in the library. The result of Blastx indicated that 26 genes had homologous sequences and 7 genes showed high similarities with putative proteins; 2 genes did not get any homologous sequence or protein. Defense-related genes (40.3%) were most in the library; The energy metabolism and the cytoprotective genes were in the next place (23.4%and 14.9%respectively). The genes related to eucaryotic cell structure, protein synthesis, transduction and signal transduction were fewer (8.5%, 4.3%, 4.3%and 4.3%respectively). DMP can induce numerous genes expression in tobacco. Masses of genes are related to plant growth and stress resistance.
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