Objective To investigate the correlation between plasma homocysteine (Hcy) and its metabolic en-zyme methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism and coronary heart disease (CHD) in Han population in Yulin area of Guangxi zhuang Autonomous Region. Methods A total of 300 Han Chinese in Yulin area were included in the study, and divided into the CHD group and the non-CHD group, the control group by coronary angiography (n=150 each). The peripheral blood was collected to extract DNA. The MTHFR C677T genotype in two groups was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Meanwhile, the Hcy level and other biochemical indicators were examined by automatic biochemical analyzer. Results There was statistical significance in the Hcy level between the two groups ( P<0.05). The frequency of the three geno-types CC, CT and TT expression in the CHD group and the control group were 18.7%, 47.3%, 34.0%and 37.3%, 42.0%, 20.7%, respectively. The frequency of C and T allele expression was 42.3%and 57.7%in the CHD group, and 58.3%and 41.7%in the control group. The overall distribution frequency and allele frequency of the three geno-types in the two groups were statistically significant (P<0.05). In the CHD group, the Hcy of TT genotype was higher than that of CC and CT genotypes (P<0.05). Logistic regression showed that age, blood pressure, and lipid level may be risk factors for CHD. Conclusion The elevated Hcy level and its metabolic enzyme MTHFR C677T gene poly-morphism may be associated with the occurrence of CHD in Han population in Yulin of Guangxi zhuang Autonomous Region.%目的：探讨血浆同型半胱氨酸（Hcy）及其代谢酶 N5，10-亚甲基四氢叶酸还原酶（MTHFR） C677T基因多态性与广西壮族自治区玉林地区汉族冠心病的关系。方法纳入300例汉族人，经冠状动脉造影确诊的冠心病（CHD）组150例，非冠心病组作为对照组150例，抽取外周静脉血提取DNA，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的方法检测2组MTHFR C677T基因型，同时用全自动生化分析仪检测Hcy及其他生化指标。结果2组Hcy水平比较差异有统计学意义（P＜0.05）。CC、CT、TT 3种基因型频率表达在CHD组和对照组中分别为18.7％、47.3％、34.0％和37.3％、42.0％、20.7％；CHD 组中 C 和 T 等位基因为42.3％和57.7％，对照组中为58.3％和41.7％。2组3种基因型总体分布频率及等位基因频率分布差异均有统计学意义（P＜0.05）。Hcy在CHD组中TT型高于CC型及CT型（P＜0.05）。Logistic回归显示，年龄、血压、血脂水平可能为冠心病发病的危险因素。结论 Hcy升高及其代谢酶（MTHFR）C677T基因多态性可能与玉林地区汉族冠心病的发生有关。
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