聚酯型儿茶素对人肝癌细胞生长的抑制作用及其机制

摘要

目的探讨聚酯型儿茶素对人肝癌SMMC-7721细胞的生长抑制作用及其机制。方法应用MTT法、集落形成试验、同位素掺入试验、细胞内 cAMP/cGMP浓度测定、原位杂交等方法进行检测。结果 50 mg*L-1聚酯型儿茶素对SMMC-7721细胞的生长、集落形成有明显的抑制作用；［3H］-TdR、［3H］-Leu掺入试验证明其可明显抑制细胞DNA和蛋白质合成；聚酯型儿茶素作用后，SMMC-7721细胞内cAMP及cGMP浓度明显增加，细胞的c-myc基因mRNA水平表达明显降低，而p53基因mRNA水平表达明显升高。结论聚酯型儿茶素对肝癌SMMC-7721细胞的生长有明显的抑制作用，此作用与其抑制细胞DNA和蛋白质合成、升高细胞内cAMP浓度和抑制c-myc基因表达、增强p53基因表达有关。%AIM　To investigate the inhibitory effects on the growth of SMMC-7721 human hepatocellular carcinoma cell line and its mechanism by theasinesin. METHODS　MTT assay,colony formation test, 3H-TdR, 3H-Leu incorporation test, determination of the intracellular cAMP and cGMP level, in situ hybridization were used. RESULTS　Theasinesin at 50 mg*L-1 could significantly inhibit the growth and colony formation of SMMC-7721 cells. Theasinesin could strongly inhibit DNA and protein synthesis and increase intracellular cAMP levels. C-myc gene expression was significantly decreased and p53 gene increased strongly in a dose-dependent manner.CONCLUSION　Theasinesin demonstrates significant inhibitory effects on the growth of SMMC-7721 cells. Its mechanisms may involve the inhibition of DNA and protein synthesis ,the elevation of intracellular cAMP levels, inhibition of c-myc gene expression and enhancement of p53 gene expression.