橘红素增强5-氟尿嘧啶抑制人胃癌AGS细胞增殖的作用及其机制研究

摘要

Aim To study the effects and mechanismsof tangeretin combined with 5-fuorouracil ( 5-Fu ) onproliferation of human AGS gastic cancer cells. Meth-ods MTT assay was used to detect the growth inhibi-tory rates of human AGS gastric cancer cells treatedwith 5-Fu and tangeretin combined with 5-Fu. The cellcycle distribution was measured by flow cytometry. Theprotein expressions of p21 and p53 were detected byWestern blot. Results MTT assay showed 5-Fu inhib-ited the growth of AGS gastric cancer cells in a dose-dependent manner ( P ＜ 0. 05 , P ＜ 0. 01 ) and inhibi-tory rates of tangeretin combined with 5-Fu was higherthan those of 5-Fu alone ( P ＜ 0. 01 ). Tangeretin com-bined with 5-Fu arrested AGS cells in S phase com-pared to control group ( P ＜ 0. 01 ). Western blotshowed that tangeretin combined with 5-Fu increasedexpressions of p53 and p21 protein compared to thattreated with 5-Fu alone ( P ＜ 0. 01 ). ConclusionsTangeretin combined with 5-Fu can enhance the prolif-eration inhibition of 5-Fu in human gastric cancer AGScells. S phase arrest and upregulation of p53 and p21protein might be the mechnisms.%目的 探讨橘红素联合5-氟尿嘧啶(5-Fu)对人胃癌AGS细胞增殖的作用及其机制.方法 采用MTT法观察5-Fu和橘红素联合5-Fu对AGS胃癌细胞增殖作用;流式细胞仪检测橘红素联合5-Fu对细胞周期的影响;Western blot检测橘红素联合5-Fu对p53和p21蛋白表达的影响.结果 5-Fu可呈剂量依赖地抑制人AGS胃癌细胞的增殖(P<0.05,P<0.01),橘红素联合5-Fu可明显增强5-Fu对AGS胃癌细胞增殖的抑制作用,与5-Fu组比较差异有统计学意义(P<0.01);橘红素联合5-Fu作用于AGS细胞,S期细胞百分比明显增高,与对照组比较差异有统计学意义(P<0.01);橘红素联合5-Fu可上调p53和p21蛋白的表达,与5-Fu组比较差异有统计学意义(P<0.01).结论 橘红素联合5-Fu可增强5-Fu抑制人AGS胃癌细胞的增殖作用,主要机制可能是通过上调p53和p21蛋白,使细胞阻滞于S期.