目的 观察不同剂量盐酸吡格列酮对STZ诱导的糖尿病大鼠肾组织氧化应激的影响.方法 STZ腹腔注射建立糖尿病大鼠模型.成模糖尿病大鼠随机分为模型组和不同剂量吡格列酮组,并设正常对照组,干预8周后检测肾皮质MDA含量,SOD活性,肾组织NADPH氧化酶亚单位p22phox mRNA和p47phox mRNA表达.结果各吡格列酮组较模型组MDA含量,p22phox mRNA和p47phox mRNA表达明显降低,SOD活性明显升高(P<0.05).结论 吡格列酮可抑制STZ糖尿病大鼠肾脏NADPH氧化酶表达,降低肾脏氧化应激水平,并具有一定的剂量依赖性,该作用可能与其肾脏保护部分有关.%Aim To observe the effect of different dosages of pioglitazone on the oxidative stress in the kidney of STZ-induced diabetic rats. Methods Diabetic models were established by a single injection of strepto-zotocin. All the diabetic rats were randomly divided into following groups: diabetic rats without treatment, different dosages of pioglitazone treated diabetic rats, healthy rats served as a normal control group. At the 8 th week, the renal tissue was acquired for measuring malonaldehyde( MDA ) level, expression of p22phox Mrna and p47phox Mrna, activities of superoxide dismutase ( SOD ) Result Compared with model group, MDA level, expression of p22phox Mrna and p47phox Mrna had decreased and SOD activity had increased significantly in pioglitazone group ( P < 0. 05 ). Conclusion Pioglitazone can inhibit NADPH oxidase expression, lower the oxidative stress in the kidney in a dose-independent manner to some extent, which may play a reno-protective role in STZ-induced diabetic rats.
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