温阳通脉方对大鼠心肌缺血再灌注RISK通路机制研究

摘要

Objective To study the effect of Wenyangtongmai Decoction on rats with myocardial ischemia/reperfusion (I/R) injury and its mechanism. Methods Forty male SD rats were randomly divided into sham operation group , ischemia reperfusion (IR) group , is-chemia preconditioning (IP) group and Wenyangtongmai decoction (WY) group.The IR and IP models were established by ligation of LAD.cTnT in serum were measured.The expression of the bax and bcl-2 was tested by immunohistochemistry method.The expression of Akt was tested by Western blot method. Results The serum level of cTnT in IP and WY group were lower than IR group , but higher than sham group.The expression level of bax in IP and WY group were lower than IR group ( P<0.05) . The expression level of akt and bcl-2 in IP and WY group were higher than IR group (P<0.05) . but there was no difference in the serum level of cTnT and the expression level of bax , bcl-2 and akt between the two treatment groups ( P>0.05) . Conclusion Wenyangtongmai decoction pre-conditioning can protect ischemia reperfusion rats by uploading the expression of Akt , bcl-2 and download the expression of Bax.%目的：观察温阳通脉方对缺血再灌注大鼠心肌的保护作用及其可能机制。方法将40只SD雄性大鼠随机分为假手术组、心肌缺血再灌注损伤组（IR组）、心肌缺血预适应组（IP组）、温阳通脉方组（WY组）。建立大鼠心肌缺血再灌注损伤（IR）模型及心肌缺血预适应（IP）模型。检测各组大鼠血清心肌坏死标记物肌钙蛋白T （cTnT）含量，免疫组化法检测心肌bax、bcl-2蛋白表达， western-blot检测akt、 p-akt表达。结果 WY组、 IP组血清cTnT含量低于IR组（P <0.05），高于假手术组（P<0.05）。 WY组、 IP组bax表达低于IR组（P<0.05）。 WY组和IP组bcl-2、 akt蛋白表达高于IR组（P<0.05）。 WY和IP组血清cT-nT含量及心肌bcl-2、 bax、 akt表达比较无统计学差异（P>0.05）。结论温阳通脉方预处理具有心肌保护作用，其保护机制可能与激活akt通路，上调bcl-2，抑制bax蛋白表达有关。