鞘氨醇激酶通过调控血管发生促进肝癌转移

摘要

目的 确定鞘氨醇激酶(SPK)在肝癌转移中的作用.方法 用 Ad-SPK1 腺病毒感染肝癌细胞 LM-3 使其 SPK过表达,Western blot 检测 SPK1 表达及激活情况;Transwell、划痕实验检测肝癌细胞迁移情况.体外管状结构形成实验检测 SPK 对脐静脉内皮细胞(HUVEC)管状化形成的影响.建立 SPK 过表达裸鼠模型,体内检测肝癌迁移情况.结果 在体外 SPK 对肝癌细胞的迁移无显著影响;Ad-SPK 能够促进 HUVEC 管状结构形成;裸鼠皮下移植瘤结果 显示 Ad-SPK 促进肿瘤生长和转移.结论 鞘氨醇激酶通过调控血管发生促进肝癌转移.%Objective To determine the role of sphingosine kinase (SPK) in hepatocyte carcinoma (HCC) metastasis.rnMethods The expression and activation of SPK in Ad-SPK infected LM-3 HCC cell line was detected by Western blot; and HCC cells migration was evaluated by Transwell and scar-healing experiments. The impact of SPK on HUVEC tube morphologenesis was examined by in vitro tube formation assay. The SPK-overexpressing nude mice model was established to access HCC metastasis in vivo.rnResults SPK did not influence significantly HCC cells migration in vitro; HUVEC tube formation was stimulate by Ad-SPK. Ad-SPK accelerated the tumor growth and metastasis in vivo.rnConclusion Sphingosine kinase promotes the HCC metastasis via regulating angiogenesis.