轮状病毒腹泻患儿病毒血症研究

摘要

Aim To explore the incidence of virusemia in childhood patientswith acute rotavirus(RV) infectious diarrhea.Methods Reverse transcriptase polymerase chain reaction(RT-PCR) was applied to detect RV in serum from 200 infants and young children with acute diarrhea（RV positive in stool confirmed by ELISA ）.Results (1)14 cases were serum RV positive at the first day of hospitalization, accompanied by obvious fever and symptoms of extra-intestinal organs damage. There was not significant difference between various age groups (P＞0.25).(2)73 patients were RV positive at the moment diarrhea disappeared.Conclusion RV virusemia may cause damage of extra-intestinal organs. The asymptomatic RV carrier after diarrhea disappearance could be one important source of infection.%目的 探讨急性轮状病毒（RV）腹泻患儿病毒血症的发生情况。方法 大便ELISA一步法证实为急性RV腹泻的不同年龄患儿200例，RT-PCR法检测其血清中RV。结果 入院当日14例血清RV阳性，不同年龄组间无显著差异（χ2=1.96，P(0.25），均伴有不同程度肠外器官损害表现和明显发热；腹泻停止后血中RV全部阴性，大便73例阳性。结论 急性RV腹泻患儿可通过病毒血症引起肠外器官损害，腹泻消失后的无症状携带者是不容忽视的传染源。