rSj26GST-Sj32融合蛋白-IgG4-ELISA诊断慢性血吸虫病的研究

摘要

目的 探讨rSj26GST-Sj32-IgG4-ELISA诊断慢性血吸虫病的价值.方法 用rSj26GST-Sj32融合蛋白作为包被抗原,通过rSj26GST-Sj32-IgG4-ELISA检测慢性日本血吸虫病患者血清抗体,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊型棘球蚴病、乙型肝炎、肺结核患者血清和健康人血清作为对照.结果 该法检测慢性血吸虫病的敏感性和特异性分别为95.00%和100.00%,且与泡型棘球蚴病、囊型棘球蚴病、乙型肝炎和肺结核患者血清均无交叉反应;诊断慢性血吸虫病的阳性预告值、阴性预告值及诊断效率分别为100.00%、95.56%和97.59%.结论 rSj26GST-Sj32-IgG4-ELISA可用于慢性血吸虫病的免疫诊断.%In order to study the value of rSj26GST-Sj32-IgG4-ELISA for diagnosis of chronic schistosomiasis, the IgG4 antibodies were observe to detect sera of chronic schistosomiasis with rSj26GST-Sj32 fusion protein by rSj26GST-Sj32-1gG4-ELISA and the sera of patients with clonorchiasis sinensis, paragonimiasis westermani, alveolar echinococcosis, cystic echinococcosis, hepatitis B and pulmonary tuberculosis as well as the healthy people were taken as controls. The sensitivity and specificity of this method were 95.00％ and 100.00％ respectively, and there was no cross reaction with the sera of patients with alveolar echinococcosis, cystic echinococcosis, hepatitis B and pulmonary tuberculosis. The positive predictive value, negative predictive value and efficiency of diagnosis were 100.00％, 95.56％ and 97.59％, respectively. It is suggested that rSj26GST-Sj32-1gG4-ELISA could be applied to immunodiagnosis for chronic schistosomiasis.