河南省SIV地方株分离鉴定及生物学特性研究

摘要

目的 进一步阐明河南省猪场猪流感病毒生物学特性.方法 用鸡胚法从河南某大型屠宰场随机采集的360份猪鼻拭子和某猪病门诊所采集有呼吸道症状的35份猪鼻拭子和肺脏脾脏样品里分离猪流感病毒.结果 成功分离到3株猪流感病毒,分别命名为:A/Swine/Henan/01/2012(H1N2),A/Swine/Henan/01/2012(H1N1),A/Swine/Henan/02/2012(H1N1).这3株病毒的生物学特性研究结果表明分离株对鸡、兔、小白鼠及牛1％红细胞都有血凝性,该分离株对乙醚、氯仿和pH3.0的盐酸这3种化学试剂敏感,并且不同毒株EID50、ELD50有所差异,感染小鼠第4d开始发病.结论 河南省猪场有不同亚型流感病毒感染,应进一步研究和关注.%To further understand the prevalence of swine influenza in pig farm in Henan Province,360 porcine nasal swabs and 35 swine lung and spleen samples of pigs that showed clinic signs of respiratory symptoms were collected from one big slaughterhouse and one clinic service of swine diseases.The chicken embryo inoculation method was used for virus isola tion,the biological characterizations of swine influenza virus were tested and analysed.The results showed that three strains of swine influenza virus were isolated successfully,two isolates were H1N1 subtype and one was H1N2 subtype,naming as:A/Swine/Henan/01/2012(H1N2),A/Swine/Henan/01/2012(H1N1),and A/Swine/Henan/02/2012(H1N1),respectively.On this basis,these isolated strains were purified by limited dilution cloning.Biological characteristics research showed that the isolates had hemagglutinin to 1％ red cells of chicken,rabbit,mouse and bovine.In addition,the isolates were sensitive to the chemical reagent of Ethyl ether,chloroform and acids of pH3.0.The biological characteristics for strains of EID50 and.ELD50 were different from each other,and the onset of the diseases in the mice was 4 days after the infection.The swine influenza is an epidemic disease in Henan,and zoonotic subtype exist in pig farm,the influence to public health need to be attached more at tention.