河南省2007-2010年小肠结肠炎耶尔森菌监测分析

摘要

In order to survey the germ-carrying of Yersinia enterocolitica in diarrhea patients and host animals in Henan Province , we analyze the serological distribution and virulence gene situation of Yersinia enterocolitica and explore the causes of disease . The specimens in Henan surveillance sites from 2007 to 2010 were identified preliminarily and then they were identified finally in Chinese Center for Disease Control and Prevention . A part of the strains were serotyped and detected for virulence gene of ail, ystA , ystB, yadA , verF, and rfbC. As a result, 273 strains of Yersinia enterocolitica were detected in 8 561 specimens in Henan Province from 2007 to 2010 and the detection rate was 3 .19% . There were statistical differences among detection rates in different years . In all the serotypes , the highest proportion was O:5 (31 .63% ), following by O:8 (17 .35% ) and O:3 (11 .22% ), whereas O:9 was not detected . The most serotypes were detected in pigs (9 types) and humans , mainly containing O:3 , O:8 and O:10 . Pathogenic strains were detected in patients , pigs, cattle and dogs . In brief, Yersinia enterocolitica existed in humans and many kinds of animals in Henan Province . The humans' serotypes there were in accord with the humans' serotypes in the whole country . In Henan Province , the important animal hosts were pigs , cattle and dogs, and they may play an important role in humans' infection .%目的 监测河南省小肠结肠炎耶尔森菌在腹泻病人和宿主动物中带菌情况,分析其血清学分布及毒力基因情况,探索疾病发生的原因.方法 对2007-2010年河南省监测点的标本进行初步鉴定,鉴定后菌株送中国疾病预防控制中心传染病所进行最后鉴定,对部分菌株进行血清分型,并进行ail、ystA、ystB、yadA、verF、rfbC毒力基因检测.结果 河南省2007-2010年共监测各类标本8 561份,检出小肠结肠炎耶尔森菌株273株,检出率为3.19%;各年份菌株检出率之间有统计学差异.血清分型中O∶5血清型占比最高(31.63%),其次为O∶8(17.35%),O∶3型占比也较高(11.22%),O∶9则未发现.猪的血清型分布最广,共涉及9个血清型,人的血清型主要为O∶3,O∶8,O∶10.毒力基因测定在病人、猪、牛、犬中检出致病菌株.结论 河南省小肠结肠炎耶尔森菌在人群和各类动物中均有存在.人群中流行的血清型与全国流行情况一致.河南省最重要的动物宿主是猪、牛、犬,其在人类感染该病中可能会起到比较重要的作用.