Objective To investigate the clinical application value of Epstein-Bar virus ( EBV ) serology ( EBNA-I-IgG, EBV-EA-D-IgG,EBV-CA-IgM, EBV-CA-IgG, EBV-CA-IgG affinity ) test in diagnosis of EBV associated diseases in children. Methods Serum samples were obtained from 129 children with EBV infection and detected with enzyme-linked immunosorbent assay( ELISA ). The results were reviewed in a retrospective analysis. Results Among these cases, 48 cases ( 37.2％ ) showed positive to EBV-CA-IgM and 81 cases showed negative. In serological EBV-CA-IgM positive group, 5 series detection indicated different stages of EBV infection including acute,early, advanced, recurrence and chronic infection periods besides EBV infection. In serological EBV-CA-IgM negative group, early stage,advanced stage and past EBV infection were also indicated, but 12.4％ of infected children were misdiagnosed. Conclusion EBV serology test can reflect the levels of antibodies in different stages after EBV infection and the stage of infection. It can reduce missed diagnosis and indicate therapy early. This method is simple and worthy of popularizing.%目的 探讨EB病毒系列抗体5项(EBNA-1-IgG、EBV-EA-D-IgG、EBV-CA-IgM、EBV-CA-IgG、EBV-CA-IgG亲合力)检测在小儿EBV感染相关疾病诊断中的临床应用价值.方法 采用固相酶联免疫吸附法检测129例EB病毒感染患儿血清样本中的EB病毒系列抗体,并对其检测结果进行统计学分析.结果 单纯检测EBV-CA-IgM阳性患儿48例,阴性81例,阳性率为37.2%.利用EB系列5项检测,在EBV-CA-IgM阳性组中,除提示为EB病毒感染外,还可分别提示为感染急性期、早期、晚期、复发期和慢性期各阶段;在EBV-CA-IgM阴性组中,也可提示为感染早期、晚期和曾经感染期,但发现阴性组中有12.4%的感染期患儿被漏诊.结论 EB病毒系列抗体检测能根据EB病毒感染不同时期的抗体水平,确定感染各期别,可减少漏诊,提早治疗,且检测方法简单,值得在小儿EBV感染相关疾病诊断中推广使用.
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