子宫腺肌病小鼠模型建立及对生育力的影响

摘要

目的：使用口服他莫西芬法建立美国癌症研究所（ ICR）小鼠子宫腺肌病模型，并对此模型生育力情况进行评估。方法取24只新生的雌性ICR小鼠，于日龄第2天时开始口服他莫西芬，于日龄42天、90天时分别进行子宫组织形态学观察，于日龄90天时与正常性成熟雄鼠进行交配，妊娠第9天取出子宫组织，剖腹观察双侧子宫胚胎着床数。结果①子宫腺肌病模型小鼠在42日龄时出现子宫内膜间质浸润，肌层发育紊乱；②模型小鼠于90日龄时出现子宫腺肌病典型表现：子宫内膜侵入肌层，但尚未达浆膜下；③体内试验发现，与正常对照小鼠相比，模型小鼠生育力明显下降（Fisher’s值为0．000，P＜0．01）。结论口服他莫西芬法可成功诱导ICR小鼠子宫腺肌病模型，此模型小鼠生育力下降，可作为生殖医学领域子宫腺肌病研究较好的动物模型。%Objective To establish adenomyosis ( AM) model of mice by oral administration of tamoxifen to Institute of Cancer Research ( ICR) mice, and to study the fertility of the AM model .Methods Twenty-four newly born ICR mice with AM were induced by oral administration of tamoxifen from day 2 after birth.The histomorphology of uterus on day 42 and 90 was observed .The models of mice with AM were mated to normal male mice on day 90, and the implantation of embryos in uterus on 9th pregnant day was observed .Results There was infiltration of stroma in the endometrium and disordered development in tunica muscularis in the uterus of model mice on day 42. There were typical manifestations of AM in model mice on day 90, endometrium infiltrating muscular layer but not reaching the subserosa . In vivo experiment, a significantly declining of fertility in AM model mice was found when they were compared with control mice (Fisher’ s value was 0.000, P<0.01).Conclusion Oral administration of tamoxifen can induce the mouse model of AM successfully .The fertility of AM mice declines significantly , and they can be taken as ideal animal models to study the disease of AM in reproductive area .