三种不同灭活方法对新城疫病毒血凝和RT－PCR试验结果的影响

摘要

为研究热灭活、甲醛、β－丙内酯（BPL）三种不同灭活方法对新城疫病毒（NDV）血凝和RT －PCR 试验结果的影响，将收获的 NDV 鸡胚尿囊液分别用浓度为0．1％～0．5％的甲醛37℃灭活24 h 或4℃灭活48 h、浓度为0．02％～0．2％的 BPL 37℃灭活9 h、60℃热灭活30～90 min，并于灭活前后进行血凝试验和 RT －PCR 检测。结果表明，用0．02％的 BPL 37℃灭活9 h 或0．1％的甲醛4℃灭活48 h，不影响 NDV 的血凝价；采用热灭活30 min 或0．02％的 BPL 37℃灭活9 h，不影响 NDV 核酸检测。本研究可为相关病原检测以及诊断制品制备过程中灭活方法的选择提供参考。%In order to investigate the influence of different inactivation methods on Newcastle disease virus (NDV)hemagglutinin (HA)test and RT -PCR assay,viral suspensions were inactivated by 0.1% ~0.5%formaldehyde for 37 ℃24 h and 4 ℃ 48 h,0.02% ~0.2%β-propiolactone (BPL)for 37 ℃ 9 h and 60 ℃water bath for 30 ~90 min,respectively.Then the suspensions were detected by HA test and RT -PCR assay. The results showed that the optimal inactivation conditions for HA were 0.02% BPL at 37 ℃ for 9 h or 0.1%formaldehyde at 4 ℃ for 48 h;for RT -PCR were 60 ℃ water bath for 30 min or 0.02% BPL at 37 ℃ for 9 h. This study provided suggestions for the selection of appropriate inactivation methods,which were used in detecting related pathogens and preparing diagnostic reagents.