2种提取椰子树RNA方法的比较研究

摘要

椰子树组织中富含多糖和酚类化合物,为了获取高质量的RNA,笔者以椰子树叶片和根部为材料,比较分析CTAB法和SDS法提取RNA的效果。结果表明：CTAB法提取椰子树组织的总RNA效果较好,能有效克服酚类物质和多糖对RNA提取的影响。经OD值检测和电泳检测,总RNA的28 S和18 S条带清晰可见,纯度较高,完整性好,无明显降解,以此RNA为模板进行RT-PCR反应,能获得特异条带,表明该RNA完全可以用于后续的分子生物学操作。%Tissue of coconut tree is rich in polysaccharides and phenolic compounds.In order to obtain high-quality RNA,comparison of SDS method and CTAB method for total RNA extraction from leaves and roots of coconut tree was conducted.The results showed that CTAB method was more suitable and effective than SDS method in the total RNA extraction of coconut trees and effectively overcame the problems caused by polyphenols and polysaccharides in the RNA extraction.As detected with OD value and gel electrophoresis,bands of 28S and 18S rRNA are clearly visible,with high purity,integrity and no degradation.With the RNAs as templates for RT-PCR reaction,specific bands were produced,indicating that the RNAs could be used for subsequent molecular biological operations.