目的 究不同声压级次声作用小鼠后脑中胶质纤维酸性蛋白(GFAP)的改变和意义。方法BALB/C小鼠暴露于16Hz，声压90dB和130dB次声。每天作用2h，分别作用1、7、14、21和28d后，采用免疫组织化学方法观察小鼠脑中GFAP的改变。结果 现90dB和130dB次声作用后，GFAP的表达主要在海马、皮质和下丘脑等区域明显增多；130dB次声作用较90dB次声作用强；在相同强度的次声作用下，作用次数的多少与脑内GFAP的改变程度呈正相关。结论 马、皮质和下丘脑等区域对次声敏感，次声的作用效应与声压级和作用时间有关；次声可以通过脑内GFAP的改变造成脑损伤，这是次声导致脑损害的重要因素之一。%Objective To study the change and significance of GFAP in the brain of the mice exposed to infrasound of different sound pressure level .Method The BALB/C mice were exposed to infrasound of 16Hz with intensity of 90dB and 130dB for 1,7,14,21and 28 days. It was for 2h every day. Then immunohistochemical technique was exployed to detect the change of GFAP in the brain of the mice. Results The expression of GFAP increased mainly in the hippocampus,cortex and hypothamus regions after infrasound of 90dB and 130dB exposed .The effect at 130dB infrasound was more than 90dB.Conclusion The extent of GFAP change is closely related to the action times under infrasound of same sound pressure level. Infrasound bring about brain injury through the change of GFAP in the brain, which is one of the important factors that infrasound cause brain injury.
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