背景:获取更合适的组织工程皮肤种子细胞可使皮肤功能得到更好的修复.目的:分离、培养表皮干细胞和毛囊干细胞,并比较两种细胞的生物学特性.方法:体外分离培养2月龄新西兰兔表皮干细胞和毛囊干细胞,取生长良好的第2,3,6代细胞观察其生物学特性.结果与结论:毛囊干细胞较表皮干细胞贴壁快,具有更高的增殖活性.免疫组化及荧光定量PCR检测显示毛囊干细胞β1整合素、角蛋白19蛋白及mRNA表达均明显高于表皮干细胞.提示作为皮肤组织工程的种子细胞,毛囊干细胞较表皮干细胞更具优势.%BACKGROUND: More appropriate skin tissue engineering seed cells can better repair skin function.rnOBEJECTIVE: To isolate and culture epidermal stem cells and follicle stem cells, and compare their biological characteristics.rnMETHODS: Epidermal stem cells and follicle stem cells of 2-month-old New Zealand rabbits were isolated and cultured in vitro. The biological characteristics of passage 2, 3, 6 cells which grew well were observed.rnRESULTS AND CONCLUSIONS: Follicle stem cells attached faster and had higher proliferative capacity than epidermal stem cells. Immunohistochemical staining and fluorescent quantitative PCR showed that the follicle stem cells showed higher protein and mRNA expression of pi integrin and K19 than epidermal stem cells. These findings suggest that follicle stem cells, as seed cells of skin tissue engineering, are superior to epidermal stem cells.
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